Brief heat shock increases stable integration of lipid-mediated DNA transfections.
نویسندگان
چکیده
1.Lin Cereghino, J. and J.M. Cregg. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24:45-66. 2.Cregg, J.M., K.J. Barringer, A.Y. Hessler, and K.R. Madden. 1985. Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5:3376-3385. 3.Cregg, J.M. and K.A. Russell. 1998. Transformation methods, p. 27-39. In D.R. Higgins and J.M. Cregg (Eds.), Methods in Molecular Methods Molecular Biology, Vol. 103: Pichia Protocols. Humana Press, Totowa, NJ. 4.Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168. 5.Dohmen, R.J., A.W.M. Strasser, C.B. Höner, and C.P. Hollenberg. 1991. An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera. Yeast 7:691-692.
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ورودعنوان ژورنال:
- BioTechniques
دوره 38 1 شماره
صفحات -
تاریخ انتشار 2005